The DNA of a freshwater jellyfish of non-native origin, specifically Asian (Craspedacusta sowerbii), has been detected in the underground waters of the Timavo River inside the Luftloch cave, recently discovered by the Adriatic Speleological Society.

This significant scientific discovery is the result of collaboration between the University of Trieste, the Adriatic Speleological Society (SAS), and the Trieste Civic Museum of Natural History.

The sampling and analyses were carried out by the research group coordinated by Chiara Manfrin of the Department of Life Sciences at UniTS, as part of a project aimed at monitoring biodiversity in subterranean environments through the use of environmental DNA (eDNA). This innovative technique makes it possible to detect genetic traces left by organisms in their habitat, allowing the identification of species that are not easily observed.

‘This method allows us, by filtering water, to detect genetic traces left by organisms in the surrounding environment, thus enabling the identification of species that are difficult to observe directly,’ explains Manfrin. ‘The results revealed the presence of Craspedacusta sowerbii DNA, a non-native species originating in China, harmless to humans but whose effects on Karst ecosystems are under study.’

The discovery follows on from previous observations of the jellyfish in the Slovenian section of the Reka-Timavo since 2016, conducted by the Trieste Civic Museum of Natural History and published in a scientific note by Nicola Bressi and Andrea Colla. The genetic confirmation obtained now further strengthens the hypothesis of the species’ presence in the underground waters of the Karst.